Human beta Amyloid 1-40 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 2.92 pg/ml (50 μl)；8.40 pg/ml (10 μl)
Standard Curve Range: 31.25~2000 pg/ml
Standard Curve Gradient: 7 Points
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0111

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Size:

96T

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Product Details

Background

β-Amyloid (1-40), Iowa mutation is a biologically active peptide. (Several mutations in the beta amyloid precursor gene lead to autosomal dominant Alzheimer's disease in many families. Iowa mutations (where Asp 23 is replaced by Asn) are associated with severe brain amyloid vascular disease (CAA). The affected individuals have a missense mutation at APP 694. The mutated beta-amyloid peptides accumulate faster and form toxic fibrils

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0446	0.0451	0.0449
31.25	0.0817	0.0770	0.0794	0.0345
62.50	0.1291	0.1349	0.1320	0.0871
125.00	0.2772	0.2812	0.2792	0.2343
250.00	0.5233	0.5129	0.5181	0.4732
500.00	1.0246	1.0388	1.0317	0.9868
1000.00	1.8213	1.8056	1.8135	1.7686
2000.00	3.3130	3.3240	3.3185	3.2736

precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	757.9	168.5	48.0	703.8	202.7	42.7
Standard deviation	40.9	7.1	1.8	22.1	5.6	2.4
Coefficient of variation（%）	5.4	4.2	3.7	3.1	2.8	5.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

spike recovery

The spike recovery was evaluated by spiking 3 levels of human beta Amyloid 1-40 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 122% with an overall mean recovery of 105%.

sample values

Sample Matrix

Sample Evaluated

Range (pg/ml)

Detectable

Mean of Detectable (pg/ml)

Serum

n.d.- 907.22

37.50

198.85

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of beta Amyloid 1-40 in this assay. No medical histories were available for the donors.

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