Human Butyrylcholinesterase ELISA Kit
Key features and details
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CAT.NO. : ARG81593
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81593-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81593-002 | Standard | 2 X 40 ng/vial | 4°C |
| ARG81593-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81593-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81593-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81593-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81593-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81593-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81593-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81593-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81593-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81593 Human Butyrylcholinesterase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Butyrylcholinesterase in serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Butyrylcholinesterase |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 312 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
| 标准范围 | 625 - 40000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.6% Inter-Assay CV: 7.2% |
| 別名 | Butyrylcholine esterase; CHE2; CHE1; Choline esterase II; EC 3.1.1.8; Acylcholine acylhydrolase; E1; Cholinesterase; Pseudocholinesterase |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BCHE |
| 全名 | butyrylcholinesterase |
| 背景介绍 | Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq, Jul 2008] |
| 生物功能 | Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. [UniProt] |
| 产品亮点 | Related products: Butyrylcholinesterase antibodies; Butyrylcholinesterase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylated. No other PTM detected (PubMed:20946535). The major N-glycan structures are of the complex diantennary type with 1 and 2 N-acetylneuraminic acid molecules (Neu5Ac) making up approximately 33% and 47% of the total N-glycans, respectively. Only low amounts of fucosylated diantennary N-glycans are detected (approximately 2%). Triantennary N-glycans with or without fucose amount to approximately 13%, whereas 5% of the total N-glycans are of the oligomannosidic or hybrid type. [UniProt] |
检测图片 (1)
ARG81593 Human Butyrylcholinesterase ELISA Kit standard curve image
ARG81593 Human Butyrylcholinesterase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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