Human CA1 / Carbonic Anhydrase 1 ELISA Kit
Key features and details
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- 偶联标记说明:
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Brand:
CAT.NO. : ARG82250
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82250-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82250-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG82250-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82250-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82250-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82250-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82250-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82250-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82250-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82250-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82250-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82250 Human CA1 / Carbonic Anhydrase 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CA1 / Carbonic Anhydrase 1 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | CA1 / Carbonic Anhydrase 1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 156 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| 标准范围 | 312 - 20000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6% Inter-Assay CV: 5.1% |
| 別名 | Carbonic anhydrase I; EC 4.2.1.1; Carbonate dehydratase I; Carbonic anhydrase B; Car1; HEL-S-11; CA-I; Carbonic anhydrase 1; CAB |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CA1 |
| 全名 | carbonic anhydrase I |
| 背景介绍 | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| 生物功能 | Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related products: Carbonic Anhydrase antibodies; Carbonic Anhydrase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82250 Human CA1 / Carbonic Anhydrase 1 ELISA Kit standard curve image
ARG82250 Human CA1 / Carbonic Anhydrase 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
参考文献
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