Human / Canine / Porcine Insulin ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG82618
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Product Details
概述
| 产品描述 | ARG82618 Human / Canine / Porcine Insulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Insulin in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Hu, Dog, Pig |
| 应用 | ELISA |
| 特异性 | This kit could assay both natural and recombinant Human/Canine/Porcine Insulin. No significant cross-reactivity or interference was observed in the following samples: Human: C-peptide, IGF1, IGF2, IL2, IL4, IL6, IL8, IL10, IL12, IL18, IL22, Insulin R, MCP1, Relaxin 1, Relaxin 2, Relaxin 3, TGF beta 1, TNF alpha and VEGF. Mouse: GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha. Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha. The cross-reactivity to human proinsulin is 4.7%. The reactivity to Canine and Porcine insulins are predicted to be worked based on sequence homology. |
| 靶点名称 | Insulin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 3.9 pmol/L |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 7.8 - 500 pmol/L |
| 样本量 | 50 µl |
| 精确 | Intra-Assay CV: 4.5% Inter-Assay CV: 4.2% |
| 別名 | IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN |
应用说明
| 检测时间 | ~ 2.5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | INS |
|---|---|
| 全名 | insulin |
| 背景介绍 | After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| 生物功能 | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: Insulin antibodies; Insulin ELISA Kits; Insulin Duos / Panels; Insulin recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82618 Human/Canine/Porcine Insulin ELISA Kit standard curve image
ARG82618 Human/Canine/Porcine Insulin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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