Human Carboxypeptidase B2 ELISA Kit
Key features and details
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- 反应物种:
- 应用:
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- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81592
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81592-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81592-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81592-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81592-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81592-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81592-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81592-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81592-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81592-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81592-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81592-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81592 Human Carboxypeptidase B2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Carboxypeptidase B2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Carboxypeptidase B2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.7% Inter-Assay CV: 6.3% |
| 別名 | Plasma carboxypeptidase B; Thrombin-activable fibrinolysis inhibitor; PCPB; Carboxypeptidase B2; TAFI; pCPB; EC 3.4.17.20; CPU; Carboxypeptidase U |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CPB2 |
| 全名 | carboxypeptidase B2 (plasma) |
| 背景介绍 | Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
| 生物功能 | Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. [UniProt] |
| 产品亮点 | Related products: Carboxypeptidase antibodies; Carboxypeptidase ELISA Kits; Carboxypeptidase recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylated. N-glycan at Asn-108: Hex5HexNAc4. [UniProt] |
检测图片 (1)
ARG81592 Human Carboxypeptidase B2 ELISA Kit standard curve image
ARG81592 Human Carboxypeptidase B2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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