Human Caspase 8 ELISA Kit
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CAT.NO. : ARG82849
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82849-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82849-002 | Standard | 2 X 5 ng/vial | 4°C |
| ARG82849-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82849-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82849-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82849-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82849-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82849-008 | 20X Wash buffer | 30 ml | 4°C |
| ARG82849-009 | TMB substrate | 12 ml (Ready to use) | 4°C (Protect from light) |
| ARG82849-010 | STOP solution | 12 ml (Ready to use) | 4°C |
| ARG82849-011 | Plate sealer | 3 strips | Room temperature |
概述
| 产品描述 | ARG82849 Human Caspase 8 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Caspase 8 in serum and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Caspase 8 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 39 pg/ml |
| 样品类型 | Serum and cell culture supernatants. |
| 标准范围 | 78 - 5000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| 別名 | Casp-8; FADD-like ICE; EC 3.4.22.61; CAP4; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; FLICE; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; Caspase-8; Apoptotic protease Mch-5; CASP-8; MCH5; ALPS2B; MACH |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CASP8 |
| 全名 | caspase 8, apoptosis-related cysteine peptidase |
| 背景介绍 | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex (PubMed:23516580, PubMed:9006941). Cleaves RIPK1 at 'Asp-325' which is crucial for limiting apoptosis and necroptosis during embryonic development (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related news: Ripoptosome & Necrosome antibody panels are launched |
| 翻译后修饰 | Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. [UniProt] |
检测图片 (1)
ARG82849 Human Caspase 8 ELISA Kit standard curve image
ARG82849 Human Caspase 8 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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