Human Cathepsin E ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
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Brand:
CAT.NO. : ARG82848
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82848-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82848-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82848-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82848-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82848-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82848-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82848-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82848-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82848-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82848-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82848-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82848 Human Cathepsin E ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cathepsin E in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Cathepsin E |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 10 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.0% Inter-Assay CV: 5.8% |
| 別名 | EC 3.4.23.34; CATE; Cathepsin E |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CTSE |
| 全名 | cathepsin E |
| 背景介绍 | This gene encodes a member of the A1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme, an aspartic endopeptidase, may be involved in antigen processing and the maturation of secretory proteins. Elevated expression of this gene has been observed in neurodegeneration. [provided by RefSeq, Nov 2015] |
| 生物功能 | May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. [UniProt] |
| 细胞定位 | Endosome. Note=The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome. [UniProt] |
| 翻译后修饰 | Glycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide. Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57. [UniProt] |
检测图片 (1)
ARG82848 Human Cathepsin E ELISA Kit standard curve image
ARG82848 Human Cathepsin E ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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