Human Cathepsin L ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82692
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82692-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82692-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82692-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82692-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82692-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82692-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82692-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82692-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82692-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82692-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82692-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82692 Human Cathepsin L ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cathepsin L in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Cathepsin L |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 32 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.7% Inter-Assay CV: 5.6% |
| 別名 | Major excreted protein; Cathepsin L; CATL; Cathepsin L1; EC 3.4.22.15; CTSL1; MEP |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CTSL |
| 全名 | cathepsin L |
| 背景介绍 | The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012] |
| 生物功能 | Important for the overall degradation of proteins in lysosomes. [UniProt] |
| 细胞定位 | Lysosome. [UniProt] |
| 产品亮点 | Related products: Cathepsin antibodies; Cathepsin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82692 Human Cathepsin L ELISA Kit standard curve image
ARG82692 Human Cathepsin L ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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