Human CCL13/MCP-4 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0199
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Size:
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Product Details
Background
Human MCP-4, also known as CCL13, encodes a 98 amino acid (aa) precursor protein with a 23 aa hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature MCP-4. Mature MCP-4 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0085 |
0.0090 |
0.0088 |
|
|
0.69 |
0.0257 |
0.0247 |
0.0252 |
0.0165 |
|
2.06 |
0.0596 |
0.0624 |
0.0610 |
0.0523 |
|
6.17 |
0.1737 |
0.1756 |
0.1747 |
0.1659 |
|
18.52 |
0.5100 |
0.4768 |
0.4934 |
0.4847 |
|
55.56 |
1.3790 |
1.2690 |
1.3240 |
1.3153 |
|
166.67 |
3.0120 |
2.9570 |
2.9845 |
2.9758 |
|
500.00 |
4.2130 |
4.3809 |
4.2970 |
4.2882 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
11.6 |
60.1 |
194.9 |
10.7 |
53.7 |
177.4 |
|
Standard Deviation |
0.4 |
1.9 |
6.6 |
0.6 |
2.3 |
9.1 |
|
Coefficient of Variation(%) |
3.1 |
3.2 |
3.4 |
5.2 |
4.3 |
5.2 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL13/MCP-4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 100%.
The recovery ranged from 80% to 120% with an overall mean recovery of 100%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 22.30-140.00 | 100 | 75.86 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL13/MCP-4 in this assay. No medical histories were available for the donors.
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