Human CCL20/ MIP3 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0028
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Size:
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Product Details
Background
CCL20, also known as MIP-3 alpha, is a chemokine that plays an important role in dendritic cell trafficking and recruitment and activation of T cells. It is upregulated in monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts following inflammatory stimulation. CCL20 signals through CCR6 which is expressed on memory T cells, B cells and bone marrow-derived dendritic cells.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0175 | 0.0195 | 0.0185 | |
| 10.97 | 0.0264 | 0.0238 | 0.0251 | 0.0066 |
| 32.92 | 0.0320 | 0.0311 | 0.0316 | 0.0131 |
| 98.77 | 0.0640 | 0.0629 | 0.0635 | 0.0450 |
| 296.30 | 0.2220 | 0.2240 | 0.2230 | 0.2045 |
| 888.89 | 0.9390 | 0.9138 | 0.9264 | 0.9079 |
| 2666.67 | 2.4702 | 2.4450 | 2.4576 | 2.4391 |
| 8000.00 | 3.9750 | 4.0220 | 3.9985 | 3.9800 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 78.1 | 395.8 | 1027.1 | 82.4 | 379.2 | 1127.4 |
| Standard Deviation | 2.4 | 14.8 | 30.9 | 4.2 | 24.1 | 74.6 |
| Coefficient of Variation(%) | 3.1 | 3.7 | 3 | 4 | 6.4 | 6.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL20 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 121% to 128% with an overall mean recovery of 115%.
The recovery ranged from 121% to 128% with an overall mean recovery of 115%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d.-67.43 | 20 | 39.61 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL20 in this assay. No medical histories were available for the donors.
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