Human CCL23/MPIF-1 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 1.10 pg/ml (50 μl)；1.66 pg/ml (10 μl)
Standard Curve Range: 4.1~1000 pg/ml
Standard Curve Gradient: 7 Points
Number of Incubations: 2
Sample Volume: 50 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0130

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Size:

96T

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Product Details

Background

Myeloid progenitor inhibitory factor (MPIF-1), also known as CK beta 8 and MIP-3, is a member of the CC chemokine subfamily that is designated CCL23. Alternative splicing of the MPIF-1 gene results in two mRNAs that encode a short (CK beta 8) and a long (CK beta 8-1) isoform of the chemokine. CK beta 8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CK beta 8 (aa 22 - 120). Additional N-terminal processing of the 99 aa residue variant can generate a 75 aa residue CK beta 8 (aa 46 - 120) that is significantly more active than the 99 aa residue variant. Similarly, CK beta 8-1 encodes a 137 aa residue precursor protein that can give rise to a 116 and a 92 aa residue chemokine. Among CC chemokine members, MPIF-1 is most closely related to MIP-5/CCL15 (67% sequence identity) and MIP-1 alpha /CCL3 (51%). MPIF-1 mRNA is most abundant in the adult lung and liver, but is also present in bone marrow, placenta, and various myelomonocytic cell lines. MPIF-1 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages. MPIF-1 binds to CCR1 with high affinity and has been shown to be a potent chemoattractant and activator of monocytes, dendritic cells, and osteoclast precursors.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0095	0.0087	0.0091
4.10	0.0230	0.0207	0.0219	0.0128
10.24	0.0500	0.0457	0.0479	0.0388
25.60	0.1250	0.1321	0.1286	0.1195
64.00	0.3903	0.3985	0.3944	0.3853
160.00	1.3330	1.2530	1.2930	1.2839
400.00	3.3930	3.7000	3.5465	3.5374
1000.00	4.3920	4.4231	4.4076	4.3985

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	19.9	99.0	302.5	21.6	107.5	319.6
Standard deviation	1.2	3.9	9.7	1.2	4.1	13.2
Coefficient of variation（%）	5.8	3.9	3.2	5.6	3.8	4.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human CCL23/MPIF-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 93% to 120% with an overall mean recovery of 107%.

Sample Values

Sample Matrix

Sample Evaluated

Range (pg/ml)

Detectable

Mean of Detectable (pg/ml)

Serum

131.30-479.56

100

371.04

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human CCL23/MPIF-1 in this assay. No medical histories were available for the donors.

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