Human CCL3/MIP-1 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
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Brand:
CAT.NO. : AEH0146
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Product Details
Background
MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils. The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0167 | 0.0161 | 0.0164 | |
| 2.74 | 0.0479 | 0.0428 | 0.0454 | 0.0290 |
| 8.23 | 0.0673 | 0.0832 | 0.0853 | 0.0689 |
| 24.69 | 0.2058 | 0.2010 | 0.2034 | 0.1870 |
| 74.07 | 0.6641 | 0.5979 | 0.6310 | 0.6146 |
| 222.22 | 1.4760 | 1.4170 | 1.4465 | 1.4301 |
| 666.67 | 2.5390 | 2.5340 | 2.5365 | 2.5201 |
| 2000.00 | 3.5360 | 3.5510 | 3.5435 | 3.5271 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 55.8 | 271.9 | 819.4 | 33.7 | 173.5 | 578.5 |
| Standard Deviation | 2.6 | 17.2 | 28.5 | 2.0 | 13.4 | 42.3 |
| Coefficient of Variation(%) | 4.7 | 6.3 | 3.5 | 6.0 | 7.7 | 7.3 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL3/MIP-1 alpha into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 94% to 114% with an overall mean recovery of 102%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 12.28-1051.89 | 100 | 224.55 |
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