Human CCL3 / MIP1 alpha ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG80167
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80167-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80167-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80167-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80167-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80167-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80167-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80167-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80167-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80167-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80167-010 | STOP solution | 12ml | 4°C |
| ARG80167-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80167 Human CCL3 / MIP1 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CCL3 / MIP1 alpha in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human MIP-1β,MIP-1δ,MIP-3α,MIP-3β |
| 靶点名称 | CCL3 / MIP1 alpha |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 8 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | MIP-1-alpha; SIS-beta; PAT 464.1; Tonsillar lymphocyte LD78 alpha protein; G0/G1 switch regulatory protein 19-1; Small-inducible cytokine A3; C-C motif chemokine 3; LD78-alpha; 4-69; Macrophage inflammatory protein 1-alpha; MIP1A; SCYA3; G0S19-1; LD78ALPHA |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CCL3 |
| 全名 | chemokine (C-C motif) ligand 3 |
| 背景介绍 | MIP1 alpha belongs to the chemokine beta family. In vitro MIP1 alpha stimulates H2O2 production in human neutrophils. MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor 1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated. |
| 产品亮点 | Related products: CCL3 antibodies; CCL3 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Immune System kit |
| 翻译后修饰 | N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells. |
检测图片 (1)
ARG80167 Human CCL3 / MIP1 alpha ELISA Kit standard curve image
ARG80167 Human CCL3 / MIP1 alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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