Human CD147/EMMPRIN ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 1.44 pg/ml (50 μl)；3.63 pg/ml (10 μl)
Standard Curve Range: 5.49~4000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0139

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Size:

96T

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Product Details

Background

The name EMMPRIN reflects this proteins extracellular metalloproteinase inducer activity. It is a cell surface protein with two extracellular Ig domains, a transmembrane domain, and a cytoplasmic domain. Its expression is often elevated in human tumor cells, which in turn promotes MMP-1, MMP-2 and MMP-3 production in melanoma cells and MMP-2 and MMP-9 production in breast cancer cells. It interacts with several types of molecules such as integrins, caveolin-1 and MCT1. TRA-1-85 is an epitope within EMMPRIN.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0094	0.0094	0.0094
5.49	0.0292	0.0303	0.0298	0.0204
16.46	0.0711	0.0747	0.0729	0.0635
49.38	0.2033	0.2031	0.2032	0.1938
148.15	0.5809	0.6104	0.5957	0.5863
444.44	1.4780	1.4830	1.4805	1.4711
1333.33	2.8760	2.7790	2.8275	2.8181
4000.00	3.9430	3.8660	3.9045	3.8951

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	112.4	569.7	1538.8	101.4	480.9	1376.3
Standard deviation	4.0	21.3	94.7	4.6	28.0	80.6
Coefficient of variation（%）	3.6	3.7	6.2	4.5	5.8	5.9

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human CD147/EMMPRIN into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 103% with an overall mean recovery of 86%.The spike recovery was evaluated by spiking 3 levels of human CD147/EMMPRIN into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 103% with an overall mean recovery of 86%.

Sample Values

Sample Matrix

Sample

Evaluated

Range

(pg/ml)

Detectable

Mean of Detectable

(pg/ml)

Serum

139.08-781.71

100

234.71

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD147/EMMPRIN in this assay. No medical histories were available for the donors.

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