Human CD152 / CTLA4 ELISA Kit
Key features and details
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CAT.NO. : ARG82605
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Product Details
概述
| 产品描述 | ARG82605 Human CD152 / CTLA4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD152 / CTLA4 in serum, plasma and cell culture supernatants. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | This kit could assay both natural and recombinant Human CD152 / CTLA4. No significant cross-reactivity or interference was observed in the following samples: Human: IFN gamma, IL1 beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL17A, IL18, IL21, IL22, IL23, MCP1, TGF beta 1, TNF alpha and VEGF. Mouse: GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha. Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha. |
| 靶点名称 | CD152 / CTLA4 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 46.9 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 93.8 - 6000 pg/ml |
| 样本量 | 50 µl |
| 精确 | Intra-Assay CV: 3.1% Inter-Assay CV: 3.7% |
| 別名 | GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12 |
应用说明
| 检测时间 | ~ 3.5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CTLA4 |
| 全名 | cytotoxic T-lymphocyte-associated protein 4 |
| 背景介绍 | This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008] |
| 生物功能 | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;. [UniProt] |
| 产品亮点 | Related products: CD152 antibodies; CD152 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. [UniProt] |
检测图片 (1)
ARG82605 Human CD152 / CTLA4 ELISA Kit standard curve image
ARG82605 Human CD152 / CTLA4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
参考文献
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ELISA / Human / Plasma
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