Human CD200R1 ELISA kit

Key features and details

Specification: 96 Test
Sensitivity: 0.1 pg/ml (50 μl)；3.09 pg/ml (10 μl)
Standard Curve Range: 6.86~5000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0087

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Size:

96T

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Product Details

Background

CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity. The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain. Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, while its ligand, CD200, is widely distributed. Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders. Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains. The CD200 R-like molecules may interact differently with CD200. The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals. CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12.

TYPICAL DATA

pg/ml	O.D.		Average	Corrected
0.00	0.0140	0.0153	0.0147
6.86	0.0195	0.0207	0.0201	0.0055
20.58	0.0312	0.0276	0.0294	0.0148
61.73	0.0620	0.0644	0.0632	0.0486
185.19	0.1621	0.1541	0.1581	0.1435
555.56	0.4406	0.4431	0.4419	0.4272
1666.67	1.2060	1.2180	1.2120	1.1974
5000.00	2.7690	2.6630	2.7160	2.7014

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	1690.5	461.7	84.6	1620.9	435.3	81.4
Standard Deviation	121.9	24.2	4.1	109.3	27.2	5.5
Coefficient of Variation(%)	7.2	5.2	4.8	6.7	6.2	6.7

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human CD200R1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 76% to 123% with an overall mean recovery of 102%.

Sample Values

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	747.49-1789.41	100	1147.83

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. Donor medical histories were not available.

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