Human CD244 recombinant protein (Fc-His-tagged, C-ter)
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CAT.NO. : ARG70265
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Product Details
概述
| 产品描述 | HEK293 expressed, Fc-His-tagged (C-ter) Human CD244 recombinant protein. |
|---|---|
| 反应物种 | Hu |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | CD244 |
| 物种 | Human |
| A.A. 序列 | Cys22 - Arg221 of Human CD244 (NP_057466.1) with an Fc-6X His tag at the C-terminus. |
| 表达系统 | HEK293 |
| 別名 | NK cell activation-inducing ligand; h2B4; Natural killer cell receptor 2B4; CD antigen CD244; 2B4; NAIL; NKR2B4; SLAM family member 4; Nmrk; SLAMF4; Signaling lymphocytic activation molecule 4; NK cell type I receptor protein 2B4 |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD48 at 5 ug/ml (100 µl/well) can bind recombinant human CD244 with a linear range of 0.2-1 ug/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD244 |
| 全名 | CD244 molecule, natural killer cell receptor 2B4 |
| 背景介绍 | This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| 生物功能 | Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor (PubMed:10359122, PubMed:8376943, PubMed:11714776). Activating function implicates association with SH2D1A and FYN (PubMed:15713798). Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10934222, PubMed:15713798). Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis (PubMed:8376943, PubMed:11714776). Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells (By similarity). Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1 (PubMed:10741393). At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (PubMed:11917118). Involved in the regulation of CD8(+) T-cell proliferation; expression on activated T-cells and binding to CD488 provides costimulatory-like function for neighboring T-cells (By similarity). Inhibits inflammatory responses in dendritic cells (DCs) (By similarity). [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein. Cell membrane. Note=Receptor engagement results in a recruitment to lipid drafts essential for the subsequent tyrosine phosphorylation of the ITSMs. [UniProt] |
| 预测分子量 | 42 kDa |
| 翻译后修饰 | N-linked glycosylation is essential for the binding to its ligand CD48. Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding. Phosphorylated by FYN and CSK on tyrosine residues following activation. Coligation with inhibitory receptors such as KIR2DL1 inhibits phosphorylation upon contact of NK cells with sensitive target cells. [UniProt] |
检测图片 (1)
ARG70265 Human CD244 recombinant protein (Fc-His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70265 Human CD244 recombinant protein (Fc-His-tagged, C-ter).
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