Human CD255 / TWEAK ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG80160
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80160-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80160-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80160-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80160-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80160-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80160-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80160-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80160-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80160-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80160-010 | STOP solution | 12ml | 4°C |
| ARG80160-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80160 Human CD255 / TWEAK ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD255 / TWEAK in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human EDA-A2, APRIL, LIGHT TNF- alpha 4-1BB/Fc Chimera, Fas Ligand TRANCE OX40 Ligand, GITR Ligand TRAIL |
| 靶点名称 | CD255 / TWEAK |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | APO3L; DR3LG; TWEAK; TNLG4A |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # O43508 Human Tumor necrosis factor ligand superfamily member 12 |
|---|---|
| 基因名称 | TNFSF12 |
| 全名 | TNF superfamily member 12 |
| 背景介绍 | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010] |
| 生物功能 | Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. [UniProt] |
| 细胞定位 | Plasma membrane; Extracellular region or secreted. [UniProt] |
| 产品亮点 | Related products: CD255 antibodies; CD255 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cancer kit; Cell Biology and Cellular Response kit; Cell Death kit |
检测图片 (1)
ARG80160 Human CD255 / TWEAK ELISA Kit standard curve image
ARG80160 Human CD255 / TWEAK ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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