Human CD276/B7-H3 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 0.34 pg/ml (50 μl)；1.64 pg/ml (10 μl)
Standard Curve Range: 6.86~5000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0101

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Size:

96T

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Product Details

Background

B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0245	0.0188	0.0217
6.86	0.0357	0.0299	0.0328	0.0112
20.58	0.0549	0.0489	0.0519	0.0303
61.73	0.1073	0.1138	0.1106	0.0889
185.19	0.2891	0.2753	0.2822	0.2606
555.56	0.7364	0.7668	0.7516	0.7300
1666.67	1.7640	1.6930	1.7285	1.7069
5000.00	3.2980	3.2950	3.2965	3.2749

precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	100.5	499.3	1636.1	93.4	457.0	1443.3
Standard deviation	5.1	22.3	81.7	4.2	18.2	4.0
Coefficient of variation（%）	5.1	4.5	5.0	4.5	4.0	3.5

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

spike recovery

The spike recovery was evaluated by spiking 3 levels of human CD276/B7-H3 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 120% with an overall mean recovery of 101%.

sample values

Sample Matrix

Sample Evaluated

Range (ng/ml)

Detectable

Mean of Detectable (ng/ml)

Serum

5.04-9.65

100

6.68

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD276/B7-H3 in this assay. No medical histories were available for the donors.

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