Human CD40 recombinant protein (ECD) (His-tagged, C-ter)
Key features and details
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CAT.NO. : ARG70332
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Product Details
概述
| 产品描述 | HEK293 expressed, His-tagged (C-ter) Human CD40 recombinant protein (ECD). |
|---|---|
| 反应物种 | Hu |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | CD40 (ECD) |
| 物种 | Human |
| A.A. 序列 | Glu21 - Arg193 of Human CD40 (NP_001241.1) with 6X His tag at the C-terminus. |
| 表达系统 | HEK293 |
| 別名 | CDw40; CD antigen CD40; Tumor necrosis factor receptor superfamily member 5; Bp50; CD40L receptor; CDW40; TNFRSF5; p50; B-cell surface antigen CD40 |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD40 at 500ng/ml (100 µl/well) can bind recombinant human CD40 Ligand（Biotinylated） with a linear range of 80-320 ng/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 97% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P25942 Human Tumor necrosis factor receptor superfamily member 5 |
|---|---|
| 基因名称 | CD40 |
| 全名 | CD40 molecule, TNF receptor superfamily member 5 |
| 背景介绍 | This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014] |
| 生物功能 | Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity). [UniProt] |
| 细胞定位 | Isoform I: Cell membrane; Single-pass type I membrane protein. Isoform II: Secreted. [UniProt] |
| 预测分子量 | 31 kDa |
检测图片 (1)
ARG70332 Human CD40 recombinant protein (ECD) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70332 Human CD40 recombinant protein (ECD) (His-tagged, C-ter).
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