Human CD47/EMMPRIN ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0136
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Size:
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Product Details
Background
CD47 (also integrin-associated protein/IAP and OA3) is a variably glycosylated, 40‑60 kDa atypical member of the Ig-Superfamily. It is expressed on almost all cell types, including erythrocytes. CD47 binds to TSP-1 and SIRP alpha, and forms a membrane complex with CD36 and alpha v beta 3. Mature human CD47 is a 305 amino acid (aa), five-transmembrane glycoprotein. It contains a 123 aa extracellular region (aa 19‑141) that is characterized by the presence of a V-type Ig-like domain (aa 19‑127), and a 34 aa C-terminal cytoplasmic tail that interacts with Gi alpha subunits. Three splice variants occur over aa 293‑323. Over aa 19‑139, human CD47 shares 61%, 71% and 66% aa identity with mouse, porcine and canine CD47, respectively.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0092 | 0.0099 | 0.0096 | |
| 6.86 | 0.0223 | 0.0214 | 0.0219 | 0.0123 |
| 20.58 | 0.0512 | 0.0474 | 0.0493 | 0.0398 |
| 61.73 | 0.1392 | 0.1363 | 0.1378 | 0.1282 |
| 185.19 | 0.4027 | 0.3906 | 0.3967 | 0.3871 |
| 555.56 | 1.1000 | 1.0768 | 1.0884 | 1.0789 |
| 1666.67 | 2.9040 | 2.7840 | 2.8440 | 2.8345 |
| 5000.00 | 4.4723 | 4.4856 | 4.4790 | 4.4694 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 106.6 | 559.2 | 2075.7 | 108.7 | 524.7 | 1653.5 |
| Standard Deviation | 3.1 | 16.8 | 73.7 | 4.0 | 20.3 | 69.5 |
| Coefficient of Variation(%) | 2.9 | 3.0 | 3.5 | 3.7 | 3.9 | 4.2 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD47 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 119% with an overall mean recovery of 112%.
The recovery ranged from 95% to 119% with an overall mean recovery of 112%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 2.42-31.11 | 100 | 9.34 |
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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