Human CDCP1 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
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Brand:
CAT.NO. : AEH0083
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Product Details
Background
CDCP1, also known as SIMA135, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 aa extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Sre kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0241 | 0.0233 | 0.0237 | |
| 31.25 | 0.0716 | 0.0819 | 0.0768 | 0.0531 |
| 62.50 | 0.1091 | 0.1151 | 0.1121 | 0.0884 |
| 125.00 | 0.1978 | 0.2099 | 0.2039 | 0.1802 |
| 250.00 | 0.3730 | 0.3911 | 0.3821 | 0.3584 |
| 500.00 | 0.7124 | 0.7447 | 0.7286 | 0.7049 |
| 1000.00 | 1.3820 | 1.4660 | 1.4240 | 1.4003 |
| 2000.00 | 2.7060 | 2.8110 | 2.7585 | 2.7348 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 10.9 | 55 | 157.3 | 10 | 49.7 | 152 |
| Standard Deviation | 0.8 | 3.0 | 8.1 | 0.5 | 2.1 | 5.5 |
| Coefficient of Variation(%) | 7.1 | 5.4 | 5.1 | 5.4 | 4.2 | 3.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CDCP1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 120% with an overall mean recovery of 108%.
The recovery ranged from 79% to 120% with an overall mean recovery of 108%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 96.09-307.20 | 100 | 196.05 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.
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