Human CHIT1 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0186
RMB Please choose
RMB Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. It is distinct from another member, known as acidic mammalian chitinase (AMC) encoded by the CHIA gene and expressed mainly in the gastrointestinal tract and lung. Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pHs and can be processed into a C-terminally truncated 39 kDa form. CHIT1 is the best biomarker in the monitoring of Gaucher disease among the three most commonly used markers that also include acid phosphatase and angiotensin-converting enzyme (ACE). CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0155 |
0.0136 |
0.0146 |
|
|
2.74 |
0.0232 |
0.0218 |
0.0225 |
0.0080 |
|
8.23 |
0.0381 |
0.0399 |
0.0390 |
0.0245 |
|
24.69 |
0.1039 |
0.0986 |
0.1013 |
0.0867 |
|
74.07 |
0.2901 |
0.3065 |
0.2983 |
0.2838 |
|
222.22 |
0.8336 |
0.9318 |
0.8827 |
0.8682 |
|
666.67 |
2.3258 |
2.3320 |
2.3289 |
2.3144 |
|
2000.00 |
4.0844 |
4.0230 |
4.0537 |
4.0392 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
47.01 |
199.00 |
780.94 |
46.20 |
201.75 |
671.27 |
|
Standard Deviation |
1.72 |
9.54 |
45.28 |
1.93 |
5.46 |
38.00 |
|
Coefficient of Variation(%) |
3.7 |
4.8 |
5.8 |
4.2 |
2.7 |
5.7 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.24-11.50 | 100 | 4.04 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CHIT1 in this assay. No medical histories were available for the donors.
New Products
