Human cIAP2 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 0.81 pg/ml (50 μl)；1.59 pg/ml (10 μl)
Standard Curve Range: 2.74~2000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0123

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Size:

96T

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Product Details

Background

Cellular inhibitor of apoptosis protein1 (cIAP-1, also known as BIRC2, MIHB, and HIAP2) and 2 (cIAP-2, also known as BIRC3, MIHC, and HIAP1), are members of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Structurally, cIAP-1 and cIAP-2 are each comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). Human cIAP-1 and cIAP-2 share 70% sequence identity. Functionally, cIAPs inhibits caspases through the direct interaction of its BIR domain with the active caspase. The ring finger domain of cIAP-1 and -2 also functions as an E3 ubiquitin ligase to ubiqutinylate specific target proteins. Caspase activity may be restored by mitochondrial proteins, such as SMAC/Diablo or HtrA2/Omi, through interactions with the Reaper-like motif and the BIR domain. cIAPs are reported to be cleaved by HtrA2/Omi.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0139	0.0145	0.0142
2.74	0.0221	0.0234	0.0228	0.0086
8.23	0.0328	0.0302	0.0315	0.0173
24.69	0.0653	0.0689	0.0671	0.0529
74.07	0.1791	0.1718	0.1755	0.1613
222.22	0.5172	0.5250	0.5211	0.5069
666.67	1.4860	1.4850	1.4855	1.4713
2000.00	3.3930	3.4200	3.4065	3.3923

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	27.3	173.6	525.6	39.3	185.7	604.2
Standard deviation	2.1	11.6	25.5	3.1	13.1	37.2
Coefficient of variation（%）	7.9	6.7	4.9	8.0	7.1	6.2

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human cIAP2 into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 102% to 119% with an overall mean recovery of 110%.

Sample Values

Sample Matrix

Sample Evaluated

Range (Pg/ml)

Detectable

Mean of Detectable (Pg/ml)

Serum

n.d.-4.39

33.3%

1.80

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human cIAP2 in this assay. No medical histories were available for the donors.

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