Human CILP ELISA Kit
Key features and details
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- 偶联标记说明:
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CAT.NO. : ARG82456
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82456-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82456-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82456-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82456-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82456-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82456-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82456-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82456-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82456-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82456-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82456-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82456 Human CILP ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CILP in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | CILP |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 50 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 93.8 - 6000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.4% Inter-Assay CV: 5.9% |
| 別名 | Cartilage intermediate layer protein 1; Cartilage intermediate-layer protein; CILP-1; HsT18872 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # O75339 Human Cartilage intermediate layer protein 1 |
|---|---|
| 基因名称 | CILP |
| 全名 | cartilage intermediate layer protein, nucleotide pyrophosphohydrolase |
| 背景介绍 | Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010] |
| 生物功能 | Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 产品亮点 | Related products: CILP antibodies; CILP ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Cleaved into 2 chains possibly by a furin-like protease upon or preceding secretion. [UniProt] |
检测图片 (1)
ARG82456 Human CILP ELISA Kit standard curve image
ARG82456 Human CILP ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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