Human CNTF ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0147
RMB Please choose
RMB Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。
Product Details
Background
Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0328 |
0.0323 |
0.0326 |
|
|
2.74 |
0.0495 |
0.0492 |
0.0494 |
0.0168 |
|
8.23 |
0.0814 |
0.0796 |
0.0805 |
0.0480 |
|
24.69 |
0.1741 |
0.1868 |
0.1805 |
0.1479 |
|
74.07 |
0.4648 |
0.4985 |
0.4817 |
0.4491 |
|
222.22 |
1.2410 |
1.3150 |
1.2780 |
1.2455 |
|
666.67 |
3.0640 |
3.2330 |
3.1485 |
3.1160 |
|
2000.00 |
4.5481 |
4.6198 |
4.5840 |
4.5514 |
Precision
|
|
Intra-assay Precision |
Inter-assay Precision |
||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
39.2 |
197.9 |
618.1 |
37.0 |
199.0 |
601.7 |
|
Standard Deviation |
1.7 |
8.3 |
30.7 |
2.2 |
14.8 |
39.7 |
|
Coefficient of Variation(%) |
4.4 |
4.2 |
5.0 |
6.0 |
7.4 |
6.6 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CNTF into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 74% to 112% with an overall mean recovery of 96%.
Sample Values
|
Sample Matrix |
Sample Evaluated |
Range (pg/ml) |
Detectable (%) |
Mean of Detectable (pg/ml) |
|
Serum |
30 |
n.d.-81.17 |
13 |
42.58 |
New Products
