Human Complement C5 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
Product Details
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0191 |
0.0181 |
0.0186 |
|
|
2.74 |
0.0494 |
0.0480 |
0.0487 |
0.0301 |
|
8.23 |
0.0899 |
0.0917 |
0.0908 |
0.0722 |
|
24.69 |
0.1898 |
0.1834 |
0.1866 |
0.1680 |
|
74.07 |
0.4362 |
0.4138 |
0.4250 |
0.4064 |
|
222.22 |
0.9751 |
0.9995 |
0.9873 |
0.9687 |
|
666.67 |
2.2140 |
2.1390 |
2.1765 |
2.1579 |
|
2000.00 |
3.8600 |
3.8120 |
3.8360 |
3.8174 |
|
Intra-assay Precision |
|
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
|
22 |
22 |
22 |
6 |
6 |
6 |
||
|
Average（pg/ml） |
17.8 |
92.6 |
287.7 |
16.6 |
85.8 |
261.2 |
|
|
Standard deviation |
0.7 |
4.5 |
15.2 |
0.7 |
4.8 |
16.9 |
|
|
Coefficient of variation（%） |
3.7 |
4.9 |
5.3 |
4.5 |
5.6 |
6.5 |
|
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
The spike recovery was evaluated by spiking 3 levels of human Complement C5 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 101% with an overall mean recovery of 92%.
|
Sample Matrix |
Sample Evaluated |
Range (μg/ml) |
Detectable % |
Mean of Detectable (μg/ml) |
|
Serum |
30 |
1.79-58.93 |
100 |
25.41 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Complement C5 in this assay. No medical histories were available for the donors.
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