Human CRP ELISA Instructions

Key features and details

Specification: 96 Test
Sensitivity: 4.26 pg/ml (50 μl)；21.23 pg/ml (10 μl)
Standard Curve Range: 13.72~10000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0076

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Size:

96T

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Product Details

Background

C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.

Typical Data

pg/ml	O.D.		Average	Corrected
0.00	0.0117	0.0092	0.0105
13.72	0.0135	0.0130	0.0133	0.0028
41.15	0.0201	0.0218	0.0210	0.0105
123.46	0.0482	0.0433	0.0458	0.0353
370.37	0.1187	0.1127	0.1157	0.1053
1111.11	0.3375	0.3373	0.3374	0.3270
3333.33	1.0260	0.9954	1.0107	1.0003
10000.00	2.7060	2.7060	2.7060	2.6956

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average （pg/ml）	3118.2	1092.8	240.7	3065.9	1129.0	259.3
Standard Deviation	137.0	68.5	10.3	135.2	26.0	15.6
Coefficient of Variation （%）	4.4	6.3	4.3	4.4	2.3	6.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human CRP into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 91% to 130% with an overall mean recovery of 109%.

Sample Values

Sample

Matrix

Sample

Evaluated

Range

(ng/ml)

Detectable

Mean of Detectable

(ng/ml)

Serum

27.88-1049.90

100

334.69

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human CRP in this assay. No medical histories were available for the donors.

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