Human CTGF/CCN2 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEHY0156
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Size:
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Product Details
Background
CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a common multimodular organization. Each protein contains an IGF-binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF has the ability to bind multiple ligands and has numerous effects on development, differentation, and disease. The C-terminal cysteine knot motif contains the heparin and low density lipoprotein receptor (LDLR) binding sites that contribute to the adhesive and mitogenic properties of CTGF.
Typical data
|
ng/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0068 |
0.0064 |
0.0066 |
|
|
4.69 |
0.0893 |
0.0926 |
0.0910 |
0.0844 |
|
9.38 |
0.2235 |
0.2204 |
0.2220 |
0.2154 |
|
18.75 |
0.4926 |
0.5205 |
0.5066 |
0.5000 |
|
37.50 |
1.0290 |
1.1290 |
1.0790 |
1.0724 |
|
75.00 |
1.9120 |
2.0470 |
1.9795 |
1.9729 |
|
150.00 |
2.9800 |
3.1140 |
3.0470 |
3.0404 |
|
300.00 |
3.5890 |
3.6480 |
3.6185 |
3.6119 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(ng/ml) |
6.3 |
31.3 |
98.9 |
6.0 |
31.1 |
101.0 |
|
Standard Deviation |
0.2 |
1.0 |
2.8 |
0.2 |
1.3 |
4.1 |
|
Coefficient of Variation(%) |
3.6 |
3.3 |
2.9 |
3.8 |
4.2 |
4.1 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CTGF/CCN2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 105% with an overall mean recovery of 96%.
The recovery ranged from 88% to 105% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.33-36.79 | 100 | 23.86 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CTGF/CCN2 in this assay. No medical histories were available for the donors.
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