Human CXCL12 / SDF1 recombinant protein (Active) (His-tagged, C-ter)
Key features and details
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- A.A. 序列:
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CAT.NO. : ARG70140
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Product Details
概述
| 产品描述 | E. coli expressed, His-tagged (C-ter) Active Human CXCL12 / SDF1 recombinant protein |
|---|---|
| 应用 | SDS-PAGE |
| 靶点名称 | CXCL12 / SDF1 |
| 物种 | Human |
| A.A. 序列 | Val24 - Asn88 |
| 表达系统 | E. coli |
| 生物活性 | Active |
| 活性说明 | Determined by its ability to chemoattract BaF3 cells transfected with human CXCR4. The ED50 for this effect is |
| 別名 | TPAR1; SDF1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; TLSF; PBSF; SDF-1; Intercrine reduced in hepatomas; IRH; hSDF-1; 3-72; SCYB12; hIRH; 3-67; Stromal cell-derived factor 1 |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | >98% (by SDS-PAGE) |
| 缓冲液 | 20 mM sodium citrate and 0.1 M NaCl (pH 4.5) |
| 复溶 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | CXCL12 |
|---|---|
| 全名 | chemokine (C-X-C motif) ligand 12 |
| 背景介绍 | This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
| 生物功能 | Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 翻译后修饰 | Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. [UniProt] |
检测图片 (1)
ARG70140 Human CXCL12 / SDF1 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70140 Human CXCL12 / SDF1 recombinant protein (Active) (His-tagged, C-ter).
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