Human CXCL13/BCA-1 ELISA Kit

Key features and details

Specification: 96 Test
Sensitivity: 0.56 pg/ml (50 μl)；5.32 pg/ml (10 μl)；
Standard Curve Range: 7.81~500 pg/ml
Standard Curve Gradient: 7 Points
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0172

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Size:

96T

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Product Details

Background

CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naïve B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer’s patch development.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0076	0.0074	0.0075
7.81	0.0260	0.0265	0.0263	0.0188
15.63	0.0515	0.0509	0.0512	0.0437
31.25	0.1190	0.1180	0.1185	0.1110
62.50	0.2836	0.2700	0.2768	0.2693
125.00	0.7076	0.6990	0.7033	0.6958
250.00	1.8240	1.8140	1.8190	1.8115
500.00	3.5660	3.6350	3.6005	3.5930

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	19.3	78.3	213.9	18.5	76.1	216.7
Standard Deviation	0.7	2.7	9.6	0.7	2.3	7.1
Coefficient of Variation(%)	3.4	3.4	4.5	3.8	3.0	3.3

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human CXCL13/BCA-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 96%.

Sample Values

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	28.44-275.19	100	71.8

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CXCL13/BCA-1 in this assay. No medical histories were available for the donors.

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