Human CXCL9/MIG ELISA Kit
Protocol Booklet 
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0015
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Size:
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Product Details
Background
CXCL9, also known as MIG, is a chemokine that is induced in macrophages and in CNS glial cells in response to IFN-gamma. CXCL9 signals through CXCR3 to induce the chemoattraction of activated T cells and tumor-infiltrating lymphocytes.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0162 | 0.0167 | 0.0165 | |
| 6.86 | 0.0222 | 0.0225 | 0.0224 | 0.0059 |
| 20.58 | 0.0352 | 0.0373 | 0.0363 | 0.0198 |
| 61.73 | 0.0766 | 0.0728 | 0.0747 | 0.0583 |
| 185.19 | 0.2182 | 0.2146 | 0.2164 | 0.2000 |
| 555.56 | 0.7838 | 0.7383 | 0.7611 | 0.7446 |
| 1666.67 | 2.4443 | 2.4961 | 2.4702 | 2.4538 |
| 5000.00 | 4.1092 | 4.1881 | 4.1487 | 4.1322 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 76.4 | 387 | 1421.7 | 78.7 | 376.7 | 1375 |
| Standard Deviation | 2.1 | 11.4 | 34.2 | 6 | 8.6 | 31.7 |
| Coefficient of Variation(%) | 2.8 | 3 | 2.4 | 7.6 | 2.3 | 2.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL9 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 97% with an overall mean recovery of 91%.
The recovery ranged from 83% to 97% with an overall mean recovery of 91%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d.-196.43 | 92 | 31.69 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-8 in this assay. No medical histories were available for the donors.
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