Human DLL1 ELISA Kit

Key features and details

  • 产品描述: ARG81868 Human DLL1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human DLL1 in serum, plasma (heparin, EDTA), urine, human milk and cell culture supernatants.
  • 反应物种: Hu
  • 应用: ELISA
  • 特异性: There is no detectable cross-reactivity with other relevant proteins.
  • 靶点名称: DLL1
  • 偶联标记: HRP
  • 偶联标记说明: Substrate: TMB and read at 450 nm.
  • 灵敏度: 39 pg/ml
  • 样品类型: Serum, plasma (heparin, EDTA), urine, human milk and cell culture supernatants.
  • Brand:
CAT.NO. : ARG81868
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Product Details
内含物
货号内含物名称包装温度
ARG81868-001Antibody-coated microplate8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81868-002Standard2 X 10 ng/vial 4°C
ARG81868-003Standard/Sample diluent30 ml (Ready to use) 4°C
ARG81868-004Antibody conjugate concentrate (100X)1 vial (100 µl) 4°C
ARG81868-005Antibody diluent buffer12 ml (Ready to use) 4°C
ARG81868-006HRP-Streptavidin concentrate (100X)1 vial (100 µl) 4°C
ARG81868-007HRP-Streptavidin diluent buffer12 ml (Ready to use) 4°C
ARG81868-00825X Wash buffer20 ml 4°C
ARG81868-009TMB substrate10 ml (Ready to use) 4°C (Protect from light)
ARG81868-010STOP solution10 ml (Ready to use) 4°C
ARG81868-011Plate sealer4 strips Room temperature
概述
产品描述ARG81868 Human DLL1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human DLL1 in serum, plasma (heparin, EDTA), urine, human milk and cell culture supernatants.
反应物种Hu
应用ELISA
特异性There is no detectable cross-reactivity with other relevant proteins.
靶点名称DLL1
偶联标记HRP
偶联标记说明Substrate: TMB and read at 450 nm.
灵敏度39 pg/ml
样品类型Serum, plasma (heparin, EDTA), urine, human milk and cell culture supernatants.
标准范围78 - 5000 pg/ml
样本量100 µl
精确Intra-Assay CV: 4.2%; Inter-Assay CV: 4.9%
別名DELTA1; H-Delta-1; Drosophila Delta homolog 1; Delta-like protein 1; DL1; Delta1; Delta
应用说明
检测时间~ 5 hours
属性
形式96 well
存放说明Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 28514 Human DLL1

Swiss-port # O00548 Human Delta-like protein 1

基因名称DLL1
全名delta-like 1 (Drosophila)
背景介绍DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008]
生物功能Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. [UniProt]
产品亮点Related products:
DLL1 antibodies; DLL1 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
翻译后修饰Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation (By similarity). Ubiquitinated; promotes recycling back to the plasma membrane and confers a strong affinity for NOTCH1. Multi-ubiquitination of LYS-613 by MIB1 promotes both cis and trans-interaction with NOTCH1, as well as activation of Notch signaling. Ubiquitinated by NEURL1B (By similarity).

Phosphorylated in a membrane association-dependent manner. Phosphorylation at Ser-697 requires the presence of Ser-694, whereas phosphorylation at Ser-694 occurs independently of the other site. Phosphorylation is required for full ligand activity in vitro and affects surface presentation, ectodomain shedding, and endocytosis.

O-fucosylated. Can be elongated to a disaccharide by MFNG. [UniProt]
检测图片 (1)
  • ARG81868 Human DLL1 ELISA Kit standard curve image

    ARG81868 Human DLL1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

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