Human EGF ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0023
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Size:
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Product Details
Background
EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0115 | 0.0121 | 0.0118 | |
| 1.37 | 0.0293 | 0.0297 | 0.0295 | 0.0177 |
| 4.12 | 0.0676 | 0.0683 | 0.068 | 0.0562 |
| 12.35 | 0.1633 | 0.1675 | 0.1654 | 0.1536 |
| 37.04 | 0.4536 | 0.4552 | 0.4544 | 0.4426 |
| 111.11 | 1.1453 | 1.1946 | 1.1700 | 1.1582 |
| 333.33 | 2.3065 | 2.4491 | 2.3778 | 2.3660 |
| 1000.00 | 3.2412 | 3.2825 | 3.2619 | 3.2501 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 13.2 | 61.3 | 217.8 | 20.4 | 105 | 347.9 |
| Standard Deviation | 0.5 | 4.1 | 6.6 | 1 | 5.3 | 19.3 |
| Coefficient of Variation(%) | 3.5 | 6.7 | 3 | 4.9 | 5.1 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human EGF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 78% to 106% with an overall mean recovery of 93%.
The recovery ranged from 78% to 106% with an overall mean recovery of 93%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 200.7-417.0 | 100 | 304.2 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-8 in this assay. No medical histories were available for the donors.
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