Human EPAS1 / HIF-2 alpha ELISA Kit
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CAT.NO. : ARG83442
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Product Details
概述
| 产品描述 | ARG83442 Human EPAS1 / HIF-2 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human EPAS1 / HIF-2 alpha in Serum, plasma and cell culture supernatants., Cell Lysates, Tissue Homogenates |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | EPAS1 / HIF-2 alpha |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 60 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants., Cell Lysates, Tissue Homogenates |
| 标准范围 | 125 - 8000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| 別名 | Basic-helix-loop-helix-PAS protein MOP2; HIF2A; HIF-1-alpha-like factor; HIF2-alpha; PAS domain-containing protein 2; Endothelial PAS domain-containing protein 1; HIF-2-alpha; MOP2; Hypoxia-inducible factor 2-alpha; bHLHe73; Member of PAS protein 2; ECYT4; HLF; Class E basic helix-loop-helix protein 73; PASD2; EPAS-1 |
应用说明
| 检测时间 | 3.5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q99814 Human Endothelial PAS domain-containing protein 1 |
|---|---|
| 基因名称 | EPAS1 |
| 全名 | endothelial PAS domain protein 1 |
| 背景介绍 | This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009] |
| 生物功能 | Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity). [UniProt] |
| 细胞定位 | Nucleus. Nucleus speckle. Note=Colocalizes with HIF3A in the nucleus and speckles. [UniProt] |
| 翻译后修饰 | In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity). In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. [UniProt] |
检测图片 (1)
ARG83442 Human EPAS1 / HIF-2 alpha ELISA Kit standard curve image
ARG83442 Human EPAS1 / HIF-2 alpha ELISA Kitresults of a typical standard run with optical density reading at 450 nm.
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