Human ERBB3 / HER3 recombinant protein (ECD) (His-tagged, C-ter)
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CAT.NO. : ARG70242
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Product Details
概述
| 产品描述 | HEK293 expressed, His-tagged (C-ter) Human ERBB3 / HER3 recombinant protein (ECD). |
|---|---|
| 反应物种 | Hu |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | ERBB3 / HER3 (ECD) |
| 物种 | Human |
| A.A. 序列 | Ser20 - Thr643 of Human ERBB3 / HER3 (NP_001973.2) with 6X His tag at the C-terminus. |
| 表达系统 | HEK293 |
| 別名 | MDA-BF-1; LCCS2; p180-ErbB3; Proto-oncogene-like protein c-ErbB-3; c-erbB3; p85-sErbB3; Tyrosine kinase-type cell surface receptor HER3; p45-sErbB3; erbB3-S; HER3; c-erbB-3; ErbB-3; EC 2.7.10.1; Receptor tyrosine-protein kinase erbB-3 |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ERBB3 at 2µg/ml (100µl/well) can bind Recombinant Human NRG1-beta1 with a linear range of 0.128-45.7 ng/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 97% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P21860 Human Receptor tyrosine-protein kinase erbB-3 |
|---|---|
| 基因名称 | ERBB3 |
| 全名 | erb-b2 receptor tyrosine kinase 3 |
| 背景介绍 | This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). May also be activated by CSPG5 (PubMed:15358134). Involved in the regulation of myeloid cell differentiation (PubMed:27416908). [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt] |
| 预测分子量 | 148 kDa |
| 翻译后修饰 | Autophosphorylated (PubMed:20351256). Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). [UniProt] |
检测图片 (1)
ARG70242 Human ERBB3 / HER3 recombinant protein (ECD) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70242 Human ERBB3 / HER3 recombinant protein (ECD) (His-tagged, C-ter).
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