Human FABP2 / Intestinal FABP ELISA Kit
Key features and details
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- 反应物种:
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- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG82624
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82624-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82624-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82624-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82624-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82624-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82624-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82624-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82624-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82624-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82624-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82624-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82624 Human FABP2 / Intestinal FABP ELISA KitPPPPP is an Enzyme Immunoassay kit for the quantification of Human FABP2 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | FABP2 / Intestinal FABP |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 31.3 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6% Inter-Assay CV: 7.2% |
| 別名 | Intestinal-type fatty acid-binding protein; I-FABP; Fatty acid-binding protein, intestinal; Fatty acid-binding protein 2; FABPI |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P12104 Human Fatty acid-binding protein, intestinal |
|---|---|
| 基因名称 | FABP2 |
| 全名 | fatty acid binding protein 2, intestinal |
| 背景介绍 | The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq, Jul 2008] |
| 生物功能 | FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related products: FABP2 antibodies; FABP2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82624 Human FABP2 / Intestinal FABP ELISA Kit standard curve image
ARG82624 Human FABP2 / Intestinal FABP ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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