Human FGF basic/FGF2/bFGF ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0024
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Product Details
Background
FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFFG binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0150 | 0.0150 | 0.0150 | |
| 2.74 | 0.0210 | 0.0220 | 0.0215 | 0.0065 |
| 8.23 | 0.0295 | 0.0310 | 0.0303 | 0.0153 |
| 24.69 | 0.0601 | 0.0550 | 0.0576 | 0.0426 |
| 74.07 | 0.1620 | 0.1457 | 0.1539 | 0.1389 |
| 222.22 | 0.5740 | 0.4956 | 0.5348 | 0.5198 |
| 666.67 | 2.2035 | 1.9879 | 2.0957 | 2.0807 |
| 2000.00 | 4.5685 | 4.4910 | 4.5298 | 4.5148 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 36.8 | 179.7 | 464.2 | 31.66 | 170.64 | 581.98 |
| Standard Deviation | 1.4 | 6.9 | 13.8 | 2.38 | 11.59 | 25.24 |
| Coefficient of Variation(%) | 3.9 | 3.8 | 3 | 7.5 | 6.8 | 4.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human FGF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 78% to 92% with an overall mean recovery of 86%.
The recovery ranged from 78% to 92% with an overall mean recovery of 86%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 5.45-24.74 | 100 | 15.01 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of HGF in this assay. No medical histories were available for the donors.
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