Human FOLR1 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 2.40 pg/ml (50 μl)；3.97 pg/ml (10 μl)
Standard Curve Range: 46.88~3000 pg/ml
Standard Curve Gradient: 7 Points
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0137

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Size:

96T

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Product Details

Background

Folate receptor α (FRα), also known as folate receptor 1 (FOLR1), belongs to the folate receptor family and are both cysteine-rich glycolipid anchor proteins. FRα is encoded by FOLR1 and is a cell surface glycoprotein with a molecular weight of 38-40KD. It was initially discovered as a folic acid-binding protein. It was later found that in addition to transporting folic acid, it is also involved in regulating the proliferation and metastasis of tumor cells. Folic acid is an important nutrient necessary for maintaining cell growth and metabolism, and it plays a key role in the synthesis of DNA and RNA.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0158	0.0153	0.0156
46.88	0.1271	0.1112	0.1192	0.1036
93.75	0.2292	0.2275	0.2284	0.2128
187.50	0.4572	0.4467	0.4520	0.4364
375.00	0.9516	0.9921	0.9719	0.9563
750.00	1.9200	2.0350	1.9775	1.9620
1500.00	3.9130	3.9100	3.9115	3.8960
3000.00	4.5199	4.5427	4.5313	4.5158

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	50.8	260.6	771.5	49.9	237.4	720.3
Standard deviation	3.5	14.6	48.5	2.7	13.0	38.8
Coefficient of variation（%）	6.8	5.6	6.3	5.3	5.5	5.4

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human FOLR1 into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 76% to 120% with an overall mean recovery of 101%.

Sample Values

Sample Matrix

Sample

Evaluated

Range

(pg/ml)

Detectable

Mean of Detectable

(pg/ml)

Serum

104.68-266.14

100

178.81

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of FOLR1 in this assay. No medical histories were available for the donors.

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