Human Free Triiodothyronine (fT3) ELISA Kit
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- 偶联标记:
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CAT.NO. : ARG82800
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Product Details
概述
| 产品描述 | ARG82800 Human Free Triiodothyronine (fT3) ELISA Kit is an enzyme immunoassay kit for the quantitative determination of Free Triiodothyronine (fT3) concentration in Human serum. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | Cross-Reactivity: L-Triiodothyronine: 100% D-Triiodothyronine: 34% Triiodothyropropionic acid: 20% Diiodo-D-thyronine: 0.5% D-Thyroxine: 0.3% L-Thyroxine: 0.9% |
| 靶点名称 | Free Triiodothyronine (fT3) |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.3 pg/ml |
| 样品类型 | Serum |
| 标准范围 | 2 - 40 pg/ml |
| 样本量 | 25 µl |
| 精确 | Intra-Assay CV: 6.7% Inter-Assay CV: 8.2% |
应用说明
| 检测时间 | 1 hour 15 min |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | L-Triiodothyronine, a thyroid hormone, circulates in blood almost completely bound (>99.5%) to carrier proteins. The main transport protein is thyroxine-binding globulin (TBG). However, only the free (unbound) portion of triiodothyronine is believed to be responsible for the biological action. Furthermore, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In individuals with normal thyroid function, as the concentrations of the carrier proteins change, the total T3 levels change in consert so that the free thriiodothyronine (fT3) concentration remains constant. Thus, measurements of fT3 concentrations correlate more reliably with clinical status than total triiodothyronine levels. For example, the increase in total triiodothyronine levels associated with pregnancy, oral contraceptives, and estrogen therapy result in higher total T3 levels while the fT3 concentration remains basically unchanged. This microplate enzyme immunoassay methodology provides the technician with optimum sensitivity while requiring few technical manipulations in a direct determination of fT3 |
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| 研究领域 | Signaling Transduction kit |
检测图片 (1)
ARG82800 Human Free Triiodothyronine (fT3) ELISA Kit standard curve image
ARG82800 Human Free Triiodothyronine (fT3) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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