Human Furin ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81480
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81480-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81480-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81480-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81480-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81480-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81480-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81480-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81480-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81480-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81480-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81480-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81480 Human Furin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Furin in cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Furin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 156 pg/ml |
| 样品类型 | Cell culture supernatants. |
| 标准范围 | 312 - 20000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.3% Inter-Assay CV: 5.8% |
| 別名 | FUR; Paired basic amino acid residue-cleaving enzyme; PCSK3; EC 3.4.21.75; PACE; Dibasic-processing enzyme; SPC1; Furin |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FURIN |
| 全名 | furin (paired basic amino acid cleaving enzyme) |
| 背景介绍 | This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| 生物功能 | Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. [UniProt] |
| 产品亮点 | Related products: Furin antibodies; Furin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation. Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. [UniProt] |
检测图片 (1)
ARG81480 Human Furin ELISA Kit standard curve image
ARG81480 Human Furin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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