Human GIF / Gastric intrinsic factor ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
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- 标准范围:
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Brand:
CAT.NO. : ARG82532
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82532-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82532-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82532-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82532-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82532-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82532-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82532-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82532-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82532-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82532-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82532-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82532 Human GIF / Gastric intrinsic factor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human GIF / Gastric intrinsic factor in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | GIF / Gastric intrinsic factor |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 10 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.3% Inter-Assay CV: 5.5% |
| 別名 | Gastric intrinsic factor; IFMH; TCN3; Intrinsic factor; INF; IF |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GIF |
| 全名 | gastric intrinsic factor (vitamin B synthesis) |
| 背景介绍 | This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq, Jul 2008] |
| 生物功能 | Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: GIF antibodies; GIF ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82532 Human GIF / Gastric intrinsic factor ELISA Kit standard curve image
ARG82532 Human GIF / Gastric intrinsic factor ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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