Human Granulysin ELISA Kit

产品描述: ARG81543 Human Granulysin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Granulysin in serum, plasma (heparin, EDTA) and cell culture supernatants.
反应物种: Hu
应用: ELISA
特异性: There is no detectable cross-reactivity with other relevant proteins.
靶点名称: Granulysin
偶联标记: HRP
偶联标记说明: Substrate: TMB and read at 450 nm.
灵敏度: 15.6 pg/ml
样品类型: Serum, plasma (heparin, EDTA) and cell culture supernatants.

CAT.NO. : ARG81543

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Size:

96 wells

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

内含物

货号	内含物名称	包装	温度
ARG81543-001	Antibody-coated microplate	8 X 12 strips	4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81543-002	Standard	2 X 10 ng/vial	4°C
ARG81543-003	Standard/Sample diluent	30 ml (Ready to use)	4°C
ARG81543-004	Antibody conjugate concentrate (100X)	1 vial (100 µl)	4°C
ARG81543-005	Antibody diluent buffer	12 ml (Ready to use)	4°C
ARG81543-006	HRP-Streptavidin concentrate (100X)	1 vial (100 µl)	4°C
ARG81543-007	HRP-Streptavidin diluent buffer	12 ml (Ready to use)	4°C
ARG81543-008	25X Wash buffer	20 ml	4°C
ARG81543-009	TMB substrate	10 ml (Ready to use)	4°C (Protect from light)
ARG81543-010	STOP solution	10 ml (Ready to use)	4°C
ARG81543-011	Plate sealer	4 strips	Room temperature

概述

产品描述	ARG81543 Human Granulysin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Granulysin in serum, plasma (heparin, EDTA) and cell culture supernatants.
反应物种	Hu
应用	ELISA
特异性	There is no detectable cross-reactivity with other relevant proteins.
靶点名称	Granulysin
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm.
灵敏度	15.6 pg/ml
样品类型	Serum, plasma (heparin, EDTA) and cell culture supernatants.
标准范围	31.2 - 2000 pg/ml
样本量	100 µl
精确	Intra-Assay CV: 6.3% Inter-Assay CV: 6.6%
別名	Lymphokine LAG-2; LAG-2; Granulysin; LAG2; Protein NKG5; T-cell activation protein 519; TLA519; D2S69E; NKG5

应用说明

检测时间	~ 5 hours

属性

形式	96 well
存放说明	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 10578 Human GNLY Swiss-port # P22749 Human Granulysin
基因名称	GNLY
全名	granulysin
背景介绍	The product of this gene is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. This protein is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
生物功能	Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis. [UniProt]
产品亮点	Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData
翻译后修饰	A 9 kDa form is produced by proteolytic processing of a 15 kDa protein. [UniProt]

检测图片 (1)

ARG81543 Human Granulysin ELISA Kit standard curve image
ARG81543 Human Granulysin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

参考文献

Granulysin-mediated reduction of PDZRN3 induces Cx43 gap junctions activity exacerbating skin damage in trichloroethylene hypersensitivity syndrome

ELISA / Cell culture supernatants / Human

Bo Jiao et al.
Ecotoxicol Environ Saf., (2024)