Human Granzyme A/GZMA ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: o.46 pg/ml (50 μl)；1.46 pg/ml (10 μl)
Standard Curve Range: 2.74~2000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0129

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Size:

96T

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Product Details

Background

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0136	0.0141	0.0139
2.74	0.0255	0.0239	0.0247	0.0109
8.23	0.0392	0.0410	0.0401	0.0263
24.69	0.0845	0.0824	0.0835	0.0696
74.07	0.2141	0.2112	0.2127	0.1988
222.22	0.6253	0.5911	0.6082	0.5944
666.67	1.7910	1.7440	1.7675	1.7537
2000.00	3.7830	3.7900	3.7865	3.7727

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	49.6	198.7	609.8	47.5	189.0	608.9
Standard deviation	2.3	8.7	26.3	1.5	7.3	20.0
Coefficient of variation（%）	4.6	4.4	4.3	3.2	3.9	3.3

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human Granzyme A into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 81% to 97% with an overall mean recovery of 91%.

Sample Values

Sample Matrix

Sample Evaluated

Range (pg/ml)

Detectable

Mean of Detectable (pg/ml)

Serum

62.47-179.55

100.0%

113.58

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human Granzyme A in this assay. No medical histories were available for the donors.

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