Human Growth Hormone ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 0.34 pg/ml (50 μl)；1.81 pg/ml (10 μl)
Standard Curve Range: 6.86~5000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0142

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Size:

96T

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Product Details

Background

Growth Hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones - GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin and leptin. The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified.Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types. GH stimulates the liver and other tissues to produce IGF-I, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-I. GH, directly or indirectly via IGF-I, can act on B cells, T cells, NK cells, macrophages, and neutrophils to exert immunomodulatory activities. In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake, and protein synthesis.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0068	0.0068	0.0068
6.86	0.0263	0.0265	0.0264	0.0196
20.58	0.0752	0.0710	0.0731	0.0663
61.73	0.2097	0.2067	0.2082	0.2014
185.19	0.5981	0.5554	0.5768	0.5700
555.56	1.4450	1.4230	1.4340	1.4272
1666.67	2.7010	2.7080	2.7045	2.6977
5000.00	3.6780	3.6520	3.6650	3.6582

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	93.2	510.8	1499.9	85.7	499.7	1279.3
Standard deviation	2.6	22.8	77.5	4.7	27.4	82.3
Coefficient of variation（%）	2.80	4.50	5.20	5.5	5.5	6.4

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human Growth Hormone into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 99% to 111% with an overall mean recovery of 107%.

Sample Values

Sample Matrix

Sample

Evaluated

Range

(pg/ml)

Detectable

Mean of Detectable

(pg/ml)

Serum

69.67-3587.61

100.0

1114.75

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Growth Hormone in this assay. No medical histories were available for the donors.

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