Human GSTA1 ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82853
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82853-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82853-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82853-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82853-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82853-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82853-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82853-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82853-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82853-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82853-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82853-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82853 Human GSTA1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human GSTA1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | GSTA1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.8% Inter-Assay CV: 6.8% |
| 別名 | EC 2.5.1.18; Glutathione S-transferase A1; GSTA1-1; GST-epsilon; GST class-alpha member 1; GTH1; GST2; GST HA subunit 1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GSTA1 |
| 全名 | glutathione S-transferase alpha 1 |
| 背景介绍 | This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
| 生物功能 | Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis (PubMed:11152686). Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (PubMed:16624487). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
检测图片 (1)
ARG82853 Human GSTA1 ELISA Kit standard curve image
ARG82853 Human GSTA1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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