Human HVEM/TNFRSF14 ELISA Instructions

Key features and details

Specification: 96 Test
Sensitivity: 1.68 pg/ml (50 μl)；1.86 pg/ml (10 μl)
Standard Curve Range: 13.72~10000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0107

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Size:

96T

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Product Details

Background

Herpesvirus entry mediator (HVEM), also referred to as TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. In the TNF superfamily nomenclature, HVEM is referred to as TNFRSF14. Human HVEM cDNA encodes a 283 amino acid (aa) protein with a probable 36 aa signal peptide, a 166 aa extracellular domain, a 23 aa transmembrane region and a 58 aa cytoplasmic region. The extracellular domain of HVEM contains several cysteine-rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor-associated factor) interaction domain. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D. Two TNF superfamily ligands, including the secreted TNF‑ beta (lymphotoxin alpha ) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LT beta R, the receptor for lymphotoxin alpha beta heterotrimer.

Typical Data

pg/ml	O.D.		Average	Corrected
0.00	0.0117	0.0092	0.0105
13.72	0.0135	0.0130	0.0133	0.0028
41.15	0.0201	0.0218	0.0210	0.0105
123.46	0.0482	0.0433	0.0458	0.0353
370.37	0.1187	0.1127	0.1157	0.1053
1111.11	0.3375	0.3373	0.3374	0.3270
3333.33	1.0260	0.9954	1.0107	1.0003
10000.00	2.7060	2.7060	2.7060	2.6956

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average （pg/ml）	3118.2	1092.8	240.7	3065.9	1129.0	259.3
Standard Deviation	137.0	68.5	10.3	135.2	26.0	15.6
Coefficient of Variation （%）	4.4	6.3	4.3	4.4	2.3	6.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human CRP into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 91% to 130% with an overall mean recovery of 109%.

Sample Values

Sample

Matrix

Sample

Evaluated

Range

(ng/ml)

Detectable

Mean of Detectable

(ng/ml)

Serum

27.88-1049.90

100

334.69

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human CRP in this assay. No medical histories were available for the donors.

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