Human IDO1 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0118
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Size:
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Product Details
Background
Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N‑formyl‑kynurenine. This degradation is the first and rate-limiting step of the L-kynurenine pathway. IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN‑ gamma, IFN‑ alpha, IFN‑ beta, and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments. Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0180 | 0.0162 | 0.0171 | |
| 6.86 | 0.0263 | 0.0268 | 0.0266 | 0.0095 |
| 20.58 | 0.0455 | 0.0428 | 0.0442 | 0.0271 |
| 61.73 | 0.0997 | 0.0941 | 0.0969 | 0.0798 |
| 185.19 | 0.2684 | 0.2517 | 0.2601 | 0.2430 |
| 555.56 | 0.7869 | 0.7186 | 0.7528 | 0.7357 |
| 1666.67 | 1.9390 | 1.8720 | 1.9055 | 1.8884 |
| 5000.00 | 3.6660 | 3.7050 | 3.6855 | 3.6684 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 119.6 | 549.6 | 1673.2 | 108.1 | 542.6 | 1597.0 |
| Standard Deviation | 5.6 | 33.6 | 90.3 | 2.9 | 22.2 | 63.4 |
| Coefficient of Variation(%) | 4.7 | 6.1 | 5.4 | 2.7 | 4.1 | 4.0 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IDO1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 103% with an overall mean recovery of 92%.
The recovery ranged from 81% to 103% with an overall mean recovery of 92%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 62.75-652.09 | 100 | 291.93 |
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