Human IFN-β ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
Product Details
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0103 |
0.0106 |
0.0105 |
|
|
15.63 |
0.0798 |
0.0747 |
0.0773 |
0.0439 |
|
31.25 |
0.1220 |
0.1323 |
0.1272 |
0.0938 |
|
62.50 |
0.2001 |
0.1991 |
0.1996 |
0.1663 |
|
125.00 |
0.3930 |
0.4183 |
0.4057 |
0.3723 |
|
250.00 |
0.8126 |
0.7841 |
0.7984 |
0.7650 |
|
500.00 |
1.7670 |
1.6420 |
1.7045 |
1.6712 |
|
1000.00 |
3.5520 |
3.5080 |
3.5300 |
3.4967 |
|
Intra-assay Precision |
|
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
|
22 |
22 |
22 |
6 |
6 |
6 |
||
|
Average（pg/ml） |
20.0 |
99.1 |
304.4 |
21.3 |
101.4 |
317.2 |
|
|
Standard deviation |
1.2 |
5.8 |
14.3 |
1.5 |
4.2 |
15.7 |
|
|
Coefficient of variation（%） |
6.2 |
5.9 |
4.7 |
7.1 |
4.1 |
5.0 |
|
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
The spike recovery was evaluated by spiking 3 levels of human IFN beta into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 102% to 129% with an overall mean recovery of 121%.
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d.-2.08 | 0.53 | 1.03 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IFN-β in this assay. No medical histories were available for the donors.
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