Human IgA Matched Antibody Pair [RM128/RM129] 31-1030-MK

Uniprot: P01876/P01877
Gene ID: 3493/3494
Host Species: Rabbit
Species Reactivity: Human
Concentration: 1) Capture Antibody: Anti-Human IgG4 Rabbit Monoclonal antibody, clone RM120, 100 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 2) Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 3) 10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise

CAT.NO. : 31-1030-MK

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Size:

Capture:100μg;Detection:25μg;Diluent:10mL;

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Product Details


Product Name	Human IgA Matched Antibody Pair [RM128/RM129]
Product Description	The Human IgA Matched Antibody Pairs is specific to Human IgA and has been demonstrated through ELISA. It does not cross-react to IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), or mouse, rat or goat immunoglobulin. The pair can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data. This product includes capture and detection antibodies and sample diluent buffer.
Catalog No.	31-1030-MK
Clone Name	RM128 Anti-Human IgA Rabbit Monoclonal Antibody RM129 Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody
Product Data Sheet	Human IgA Matched Antibody Pair
Protocol	Sandwich ELISA Protocol
Specificity	The antibody pair detects only human IgA. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses.
Application	Sandwich ELISA, or other Sandwich Assays
Species Reactivity	Human
Conjugate	RM129: Biotin
Intended Use	Research Use Only
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Properties

Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Components	1)Capture Antibody: Anti-Human IgA Rabbit Monoclonal antibody, clone RM128, 100 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 2)Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 3)10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Keywords
Usage	ELISA: Capture Antibody 50 ng/well – 200 ng/well; Detection Antibody 0.1 µg/mL – 0.5 µg/mL
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Product Images

	Sandwich ELISA using RM128 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (k+l) antibody RM129 (0.2 µg/mL) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
	Sandwich ELISA, using RevMAb human IgA matched antibody pair (capture: RM128; detection: Biotin-RM129), shows species reactivity to human only, and shows no cross-reactivity to monkey (Cyno or Rhesus), mouse IgA, rat IgA, or goat IgA.
	Sandwich ELISA, using RevMAb human IgA matched antibody pair, shows no cross-reactivity with Human IgG, IgE, IgM, or IgD.The plate was coated with human IgA capture antibody (50 ng/well).Different immunoglobulin samples (0.2 µg/mL) were added and biotinylated RM129 (0.2 µg/mL) was used as detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

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